New test rapidly detects multiple pathogens in food

by Meat&Poultry Staff
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KENT, Ohio – Crystal Diagnostics has announced its new Crystal Diagnostics MultiPath System with a demonstration Oct. 26 at the company's manufacturing facility in the Kent State Centennial Research Park. In development since 2006, the technology uses liquid crystals to detect multiple harmful pathogens in food in a single test, offering significant time savings over the most common testing methods, the company said.

Crystal Diagnostics is the exclusive licensee of fundamental liquid crystal biosensor technologies developed jointly by a research partnership between Kent State Univ. and Northeast Ohio Medical Univ., previously known as the Northeastern Ohio Universities Colleges of Medicine and Pharmacy.

A rapid, reliable method that can test for multiple pathogens simultaneously gives food-safety assurance to farmers, food processors, distributors, retailers and ultimately to the public, the company said.

Crystal Diagnostics technology includes two pieces of equipment. The first is a "cassette" containing five individual "cells," two of which are control cells and three are test cells. A prepared (enriched) sample – from ground beef or lettuce, for example – is mixed with liquid crystals and an antibody or antibody "cocktail" for the pathogen or pathogens being sought. The second piece is a "reader." The cassette is inserted into the reader. If the pathogen or pathogens being sought are present, the liquid crystal – aligned in the reader – will be disrupted. The reader recognizes this disruption and displays the result on an iPad or other device in less than 30 minutes.

In addition to speed, this technology offers other significant advantages:

  • A single test can detect multiple pathogens, for example, E. coli O157:H7 plus the other "Big Six" E. coli strains and the strain that appeared in Germany in early 2011. This capability is particularly relevant because the Food Safety and Inspection Service has announced it will require testing for E. coli strains other than O157:H7 beginning next year in beef trim used in ground beef.
  • In addition to these strains of E. coli, by utilizing the three cells in the cassette independently, the test can detect Salmonella and another pathogen, such as Listeria, in the same test.
  • The nature of the technology significantly reduces false positives and negatives even before the built-in protection of the two control cells is considered, the company said. False positives require longer product holds while retesting is accomplished; false negatives represent a different and a vastly more serious problem for food producers.

"The need for faster yet highly reliable processes to detect pathogens has never been higher given the recent deadly food outbreaks," said Paul Repetto, CEO of Crystal Diagnostics. "In 2011, a new strain of E. coli appeared in Germany sickening thousands and killing some. Millions of pounds of meat have been recalled at great expense, and most recently 25 people died from Listeria contaminated melons. This new technology can have a profound impact on public health."

The equipment will be further beta-tested in the field with leading food processing companies and laboratories this fall.

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